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Fisher Scientific 1,2-distearoyl-sn-glycero-3-phosphoethanolamine)-polyethylene glycol-n-hydroxysuccinimidyl ester (dspe-peg-nhs
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KEY RESOURCES TABLE

Journal: Cell chemical biology

Article Title: Targeted Delivery of Antigen to Activated CD169 + Macrophages Induces Bias for Expansion of CD8 + T cells

doi: 10.1016/j.chembiol.2018.10.006

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies (all monoclonal rat anti-mouse except where noted) α-CD3 (clone: 17A2) Biolegend Cat.# 100234 α-CD4 (clone: GK1.5) BD Biosciences Cat.# 561828 α-CD8a (clone: 53-6.7) Biolegend Cat.# 100705 α-CD11b (clone: M1/70) Biolegend Cat.# 101225 α-CD16/32 (Fc receptor block) (clone: 93) Biolegend Cat.# 101319 α-CD19 (clone: 6D5) Biolegend Cat.# 115545 α-CD80 (clone: 16-10A1, hamster anti-mouse) BD Biosciences Cat.# 563687 α-CD86 (clone: GL-1) Biolegend Cat.# 105011 α-CD45.1/Ly5a (clone: A20, mouse anti-mouse) Biolegend Cat.# 110713 α-CD45.2/Ly5b (clone: 104, mouse anti-mouse) Biolegend Cat.# 109805 α-CD169 (clone: MOMA-1) Bio-Rad Cat.# MCA947F α-F4/80 (clone: T45-2342 BD Biosciences Cat.# 565411 α-iNOS (clone: CXNFT) eBioscience Cat.# 12–5920-80 α-Ly6C (clone: HK1.4) Biolegend Cat.# 128011 α-Ly6G (clone: 1A8) Biolegend Cat.# 127633 α-NK1.1 (clone: PK136, mouse anti-mouse) Biolegend Cat.# 108737 α-TCR Vα2 (clone: B20.1) Biolegend Cat.# 127807 IgG2a, k isotype control Biolegend Cat.# 400507 Chemicals, Peptides, and Recombinant Proteins 1,2-distearoyl- sn -glycero-3-phosphocoline (DSPC) Avanti Polar Lipids Inc. SKU 850365P 1,2-distearoyl- sn -glycero-3-phosphoethanolamine-N-[amino(polyethylene glycol)-2000] (ammonium salt) (DSPE-PEG) Avanti Polar Lipids Inc. SKU 880128P 3-(N-succinimidyloxyglutaryl)aminopropyl polyethyleneglycol-carbamyl distearoylphosphatidyl-ethanolamine (NHS-PEG-DSPE) NOF America Corp. DSPE-034GS 9- N -(4 H -thieno[3,2- c ]chromene-2-carbamoyl)-Neu5Ac α 2–3Gal β 1–4GlcNAc-PEG-DSPE (CD169L) This study N/A AF555-PEG-DSPE Prof. Matthew Macauley N/A Cholesterol MilliporeSigma SKU C8667 Lipopolysaccharide (LPS) ( E. coli 0127:B8) MilliporeSigma SKU L3129 Chicken ovalbumin (OVA) Worthington Biochemical Corp.

Techniques: Blocking Assay, Recombinant, Plasmid Preparation, Software